Fig. 6
From: YTHDF1 targets the chemotherapy response by suppressing NOTCH1-induced stemness in colorectal cancer
YTHDF1 knockdown overcomes chemoresistance in CRC. a and b IC50 values of oxaliplatin and 5-fluorouracil in CSCs and impaired chemoresistance induced by shYTHDF1. c Effects of oxaliplatin and 5-fluorouracil on the self-renewal capacity of YTHDF1-knockdown CSCs (seeded at 1, 2, 5, and 10 cells per well for 7 days) and d spheroid formation ability (n = 5 per group). Scale bar = 20 μm. e Apoptosis assay of YTHDF1-knockdown CSC28 and POP66 cells treated with oxaliplatin and 5-fluorouracil (n = 5 per group). f Expression of apoptosis markers in YTHDF1-knockdown CSCs treated with oxaliplatin and 5-fluorouracil. g Expression of DNA damage markers in CSC28 with YTHDF1 knockdown treated with oxaliplatin and 5-fluorouracil. The data are presented as the means ± SDs. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; nonlinear regression (a, b); ELDA extreme limiting dilution analysis (c); two-way ANOVA (d, e)
