Fig. 2
From: Scarless circular mRNA-based CAR-T cell therapy elicits superior antitumor efficacy
Robust protein expression of synthesized real-scarless cmRNAs in vitro and in vivo. a Schematic illustrations depicting the architectural configurations of linear RNA, scarred circular mRNA, and scarless circular mRNA. b Expression dynamics of GLuc mRNA in linear, scarred circular, and scarless circular mRNA formats in HEK-293T cells. c, d Expression of hEPO and EGFP scarless cmRNAs in HEK-293T cells was observed via fluorescence microscopy (c) or ELISA (d). e Illustration of the formulation process of LNPs encapsulating scarless cmRNA using a microfluidics mixer. f Size distributions of LNP-cmRNA particles for scar cmRNA (left) and scarless cmRNA (right). g The expression of GLuc cmRNA in mice after intramuscular injection of 5 µg of LNP-cmRNA per mouse was measured via bioluminescence imaging. h The fluorescence intensities of the mice in (g) are presented. The error bars indicate the SEM (two-way ANOVA; *P < 0.05; ns, not significant)
