Fig. 1 | Signal Transduction and Targeted Therapy

Fig. 1

From: Inhibin beta A drives colorectal cancer progression through macrophage M2 polarization and mitochondria-dependent ferroptosis suppression

Fig. 1

INHBA upregulation in CRC correlates with advanced disease and adverse prognosis. a Representative IHC images of INHBA in 96 CRC tissues and 74 matched normal adjacent tissues. Scale bar: 500 μm (top), 200 μm (bottom). b INHBA expression in CRC vs adjacent normal tissue (top) and Kaplan–Meier OS curves (bottom; log-rank test). c In vivo tumorigenesis experiments: Subcutaneous tumor implantation experiments in C57BL/6 mice using MC38 cells stably overexpressing or knocking down INHBA. Photographs of tumors removed from euthanized mice. d Tumor growth curve (measured every 5 days) and final weight (day 20); n = 5. e Representative EdU proliferation images. Scale bar: 100 μm. f Quantification of EdU-positive cells (n = 3). g Representative colony images. h Colony number quantification (n = 3). i Representative images of migration and invasion assays with INHBA overexpression. Scale bar: 100 μm. j Quantification of migrated/invaded cells (n = 3). k Representative migration and invasion images after INHBA knockdown. Scale bar: 100 μm. l Quantification of migrated/invaded cells (n = 3). For all the statistical plots, values are expressed as the means ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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