Fig. 2 | Signal Transduction and Targeted Therapy

Fig. 2

From: Selective depletion of tumor-associated SAMHD1 enhances chemotherapeutic efficacy and antitumor immune responses

Fig. 2

Characterization of the binding between SAMHD1 and HSP90. a Identification of HSP90 as a novel SAMHD1-binding protein. HEK293T cells were transfected with SAMHD1-HA expression vector or empty control vector. Cell lysates were prepared 36 h post-transfection, and HA-tagged SAMHD1 was immunoprecipitated using HA-immobilized agarose beads. The samples were then subject to MS analysis. HSP90 was found to be one of the proteins bindings to SAMHD1. b–e Confirmation of endogenous SAMHD1-HSP90 interaction in AML-derived cells. The endogenous HSP90-SAMHD1 interaction was analyzed by co-IP assays using anti-HSP90 or control IgG antibody with samples from b THP-1 and c Molm-13. The endogenous SAMHD1-HSP90 interaction was analyzed by co-IP assays using anti-SAMHD1 or control IgG antibody with samples from d THP-1 and e Molm-13 (n = 3)

Back to article page