Fig. 7
Keratinocyte DKK3 promotes profibrotic macrophage polarization in vivo. a Wild-type (WT), DKK3 global knockout (DKK3-/-), and keratinocyte-specific DKK3 knockout (K14 Cre X Dkk3fl/fl) mice were subjected to 20 Gy thoracic irradiation. Four weeks post-irradiation, skin cells were isolated from irradiated (IR) and nonirradiated (N) skin regions and analyzed by flow cytometry, gating for CD163high and CD206high macrophages (the gating strategy is shown in supplementary Fig. 15). The ratio of the number of macrophages with the indicated surface marker expression in irradiated versus nonirradiated skin (IR/N) is shown. a Polarization markers in DKK3 global knockout (DKK3-/-) mice compared with WT mice. b Keratinocyte-specific DKK3 knockout (K14) mice compared with WT mice. Different mice presented different frequency distributions of cell subsets before treatment. Ratios of cell subset frequencies between the nontreated (NR) and treatment (IR) groups were used to allow an efficient summation of different mouse data. The data are presented as the means ± SEMs. Statistical analysis was performed via Student’s t test, *P < 0.05 compared with WT. Figure 7 was created in BioRender. Huber, P. (2025) https://BioRender.com/jpaliv7
