Fig. 6

MIF-CD44 expression pattern in PDAC patients with MASLD. a Metastatic PDAC tissues from patients were stained with HE, MIF by IHC, and a 5-color panel including CD68, CD163, CD206, CD44, CK19 by mIHC (n = 3 for Ctrl group and MASLD, respectively). Tumor area was indicated by red arrow and non-tumor area was labeled by black arrow on HE slides. b The expression of MIF was assessed by Image J based on whole-slide scanning and quantification. c, d The ratio of CK19⁺CD44⁺ cells and CD163⁺CD68⁺ cells were quantified by Halo software using HighPlex FL v4.2.14 module based on whole-slide scanning. e Proximity analysis between CD44⁺CK19⁺ cells and CD68⁺ cells were performed on the whole slide of metastatic liver tissues from PDAC patients using Halo software. Data are shown as mean ± SD per group. Unpaired parametric Student’s t-test was performed to identify differences between two groups. For proximity analyses, two-way ANOVA followed by Šídák’s multiple comparison test was performed to identify differences between two groups at different distances. A p-value less than 0.05 was considered statistically significant. *p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001