Fig. 4: Myeloid Mas orchestrates spatiotemporal metabolic reprogramming of FN1+CCR2+ monocytes. | Signal Transduction and Targeted Therapy

Fig. 4: Myeloid Mas orchestrates spatiotemporal metabolic reprogramming of FN1+CCR2+ monocytes.

From: Myeloid Mas drives pyruvate kinase M2-mediated Spi1 lactylation to fuel inflammatory senescence in MASLD

Fig. 4: Myeloid Mas orchestrates spatiotemporal metabolic reprogramming of FN1+CCR2+ monocytes.The alternative text for this image may have been generated using AI.

a–g scRNA-seq analysis of livers from HFD-fed Mas1f/f and LysMcreMas1f/f mice (n = 1 per group). a UMAP visualization of MPs. b Violin plots showing the expression of marker genes across MP clusters. c Pseudotime trajectory analysis of MP clusters. d GO-BP enrichment analysis of the Monocytes-Fn1 cluster. Cell-chat analysis of MPs presented as (e) interaction network, (f) chord diagram, and (g) heatmap. Representative immunofluorescence staining (left; scale bar = 20 μm) and quantification (right) of PKM2 (h) and γ-H2AX (i) in liver FN1⁺CCR2⁺ monocytes and F4/80⁺ macrophages (n = 5 per group). Data are shown as the mean ± SD (unpaired Student’s t test, **P < 0.01)

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