Fig. 5
Identification of human gut-associated genes in the ɸB124-14 genome. The representation of each ɸB124-14 ORF in all data sets was used to assess the consistency of the human gut-associated ecogenomic signal across the phage genome, and identify ORFs with human gut affiliations. a Average relative abundance (hits/Mb), and representation of ɸB124-14 ORFS across all 840 data sets examined. Colours of bars indicate the % of data sets with at least one valid hit to each ORF as described in the associated legend. Significant differences in average relative abundance for ORFs represented in 50% of more of the data sets examined are shown by symbols above bars and colours indicate significance vs. all other ɸB124-14 ORFs, or significance vs. all other ɸB124-14 ORFs with less than 50% representation in data sets examined. Bars show SEM. b Heatmap showing relative abundance of individual ɸB124-14 ORFs in each metagenomic data set examined. Columns represent ORFs as indicated on a x-axis, and rows represent metagenomic data sets. The intensity of shading of each cell represented the relative abundance (hits/Mb) of each ORF in each particular metagenome, corresponding to the scale provided. c Relative representation of ɸB124-14 ORFs in human gut-derived viral data sets compared to other viromes. Points show the average relative abundance of each ORF in viral metagenomes from each category, expressed as Log10 hits/Mb. Membership of each ORF with previously described functional gene clusters in the ɸB124-14 genome [18] is indicated below the x-axis. Symbols above points indicate significantly greater relative abundance in human gut viromes compared with either all other viromes, or compared with those of environmental origin. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Details of data sets in each group are provided in Supplementary Table S1
