Table 1 Microcosm setup and carbon substrate amendment

From: Methanol consumption drives the bacterial chloromethane sink in a forest soil

Carbon source added

Total carbon added (mM)a

Gas phase amendmentb

Liquid phase amendmentc

[13C]-CH3Cl

18

[13C]-CH3Cl

H2O

[13C]-CH3Cl and CH3OH

36

[13C]-CH3Cl

CH3OH

CH3Cl and [13C]-CH3OH

36

CH3Cl

[13C]-CH3OH

[13C]-CH3OH

18

–

[13C]-CH3OH

CH3Cl

18

CH3Cl

H2O

CH3Cl and CH3OH

36

CH3Cl

CH3OH

CH3OH

18

–

CH3OH

Control

–

–

H2O

  1. aAdded per pulse (5 pulses in total)
  2. bA volume of 6 mL CH3Cl was added for each CH3Cl pulse. At each pulse, a total gas phase volume of 56 mL (air with or without CH3Cl) was added to 500 mL flasks in order to maintain overpressure in the flasks
  3. cEither 1 mL milliQ water or 216 mM CH3OH stock solution was added per pulse to each microcosm
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