Table 4 Diversity indices for 16S rRNA gene OTUs obtained from heavy and light fractions of SIP experiment

Microcosm	SIP fraction	Sobs^a	Shannon index^a	Simpson diversity^a
[¹³C]-CH₃Cl	H	1762	5.95	44
[¹³C]-CH₃Cl	L	2870	7.82	223
[¹³C]-CH₃Cl and CH₃OH	H	1629	6.11	36
[¹³C]-CH₃Cl and CH₃OH	L	3150	7.55	354
CH₃Cl and [¹³C]-CH₃OH	H	1566	6.52	120
CH₃Cl and [¹³C]-CH₃OH	L	3062	7.86	283
[¹³C]-CH₃OH	H	1215	4.58	7
[¹³C]-CH₃OH	L	3148	7.40	322
Unamended control	H	1804	6.45	102
Unamended control	L	2904	7.39	217

^aCalculated at the 98% sequence identity level. See Materials and Methods for definitions. Simpson diversity is considered a conservative measure of the effective number of phylotypes [63]

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