Fig. 6
Characterization of putative tetrapyrrole precursor salvagers a Steps in cobamide biosynthesis. The enzymes that catalyze each step are indicated to the right of each arrow. The number of genomes in the complete filtered data set in each precursor salvage category is on the left. Two genomes had cobamide biosynthesis pathways inconsistent with simple auxotrophy (*). Specific tetrapyrrole precursor salvager genomes are listed in Supplementary Table 10. b HPLC analysis of corrinoid extracts from Clostridium scindens, Clostridium sporogenes, and Treponema primitia grown with and without added ALA. A cyanocobalamin standard (200 pmoles) is shown for comparison. Asterisks denote peaks with ultraviolet–visible (UV–Vis) spectra consistent with that of a corrinoid. c T. primitia ZAS-2 growth in 4YACo medium with and without added cyanocobalamin (37 nM) or ALA (1 mM). Each point represents the average of three biological replicates. Error bars are the standard deviation
