Fig. 5 | The ISME Journal

Fig. 5

From: Unveiling the role of Gardnerella vaginalis in polymicrobial Bacterial Vaginosis biofilms: the impact of other vaginal pathogens living as neighbors

Fig. 5

Quantification of the transcription of known virulence genes in G. vaginalis cultured under dual- and mono-species biofilms. a Quantification of vaginolysin (vly) transcription. b Quantification of sialidase (sld) transcription. c Quantification of HMPREF0424_0821 transcript, which encodes type II glycosyl-transferase. d Quantification of HMPREF0424_1122 transcript, which encodes a multidrug ABC transporter. e Quantification of HMPREF0424_0156 transcript, which encodes Bacitracin transport, ATP-binding protein BcrA. f Quantification of HMPREF0424_1196 transcript, which encodes a Rib-protein. The data indicate the fold-change expression of genes in G. vaginalis dual-species compared to mono-species G. vaginalis biofilm cells. For qPCR experiments, the bars represent the mean and the error bars the standard error of the mean (mean ± s.e.m.). * Values are significantly different between the dual-species consortium and the mono-species G. vaginalis biofilm under the same conditions (non-parametric Mann–Whitney U, P < 0.05)

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