Fig. 6

Atrazine degradation pathway adapted from the literature [5, 46, 68]. The relative abundance of proteins (see Table 1) is indicated in the colored rows, where multiple putative proteins catalyzing the same step are indicated as multiples rows. Three pair-wise comparison groups were created as indicated by the numbers 1, 2, 3. 1: RET vs. CHEM; 2: D0.018 vs. CHEM; 3: RET vs. D0.018. RET represents retentostats under full mass transfer limitation. CHEM represents the combined data from chemostats at D_high + D_medium—0.023, 0.032, 0.056 h⁻¹ (under no mass transfer limitation). D0.018 represents chemostats at D_low—0.018 h⁻¹ where isotope fractionation indicated the onset of mass transfer limitation. Different colors represent the log fold change (LFC) in specific proteins from a pair-wise comparison between chemostats at different dilution rates and retentostats. For example, a red field in comparison group 1 means that a protein was in higher abundance in retentostats than in chemostats at 0.023, 0.032, 0.056 h⁻¹. Symbols (*) in red indicate proteins of significantly higher abundance and in green of significantly lower abundance in the respective pair-wise comparison, where the criteria for significant differences were a p-value of <0.05 together with a cut off log fold change (LFC) of log₂(2.5). D : dilution rates, GIPA: gamma-glutamyl-isopropylamide, GALO: gamma-glutamyl-l-alaninol