Fig. 3

Phylogenetic placement of all enriched SAGs and Co-assembled SAGs (CoSAGs) in a whole genome tree based on concatenated alignments of 56 conserved marker genes. Cellulose-captured SAGs are represented by black branches, while gray branches represent a dereplicated version of the IMG reference database. References include roughly 600 genomes, reduced from ~60,000 using RNA polymerase beta-subunit clustering at 65% similarity. Symbols refer to the presence of at least one active cellulase (diamonds) or no active cellulases (circle) determined via the NIMS-Oxime assay (Supplementary Fig. 8). The bubbles note the enrichment factor which corresponds to the same taxa shown in Fig. 2. Only SAGs of high taxonomic confidence whose placement agreed with its CheckM and/or 16S rRNA gene-based taxonomy were included. The candidate phylum Goldbacteria representative Candidatus ‘Cellulosimonas argentiregionis’ CoSAG, and the highly enriched co-sorted bacterium Elusimicrobia GBS-CoSAG_05 are displayed with purple and orange branches, respectively