Fig. 1: Mycobacterium bovis resists phagocytosis and killing by D. discoideum. | The ISME Journal

Fig. 1: Mycobacterium bovis resists phagocytosis and killing by D. discoideum.

From: Mycobacterium bovis uses the ESX-1 Type VII secretion system to escape predation by the soil-dwelling amoeba Dictyostelium discoideum

Fig. 1

a The percentage of D. discoideum that have phagocytosed E. coli K12-FITC or M. bovis BCG-FITC after a 2 h incubation at an MOI of 10 was determined by flow cytometry. The mean of three biological replicates ± SEM is displayed. b Representative micrograph of D. discoideum demonstrating internalised M. bovis BCG-FITC (green—M. bovis BCG-FITC; red—actin; blue—nuclei). D. discoideum was infected with an MOI of 10 with (c) M. marinum M strain, (d) M. smegmatis mc2155 strain, or (e) M. bovis AF2122/97. Cfu of bacteria per culture of 2 × 107 amoebae was enumerated at the point of infection and after 2 days to determine bacterial survival. Streptomycin was included in the medium post infection to inhibit extracellular replication of bacteria. The mean and SEM of n = 3 independent experiments is displayed.

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