Fig. 2: Mycobacterium bovis slowly replicates for up to 7 days at 25 °C. | The ISME Journal

Fig. 2: Mycobacterium bovis slowly replicates for up to 7 days at 25 °C.

From: Mycobacterium bovis uses the ESX-1 Type VII secretion system to escape predation by the soil-dwelling amoeba Dictyostelium discoideum

Fig. 2

a Growth of M. bovis AF2122/97 was compared at 25 and 37 °C by optical density (OD600 nm) at 0 (n = 4), 4 (n = 3), 7 (n = 4) and 14 (n = 2) days. The mean and SEM of independent experiments (each performed in biological duplicate or triplicate) is displayed. Growth was compared with day 0 by two-way ANOVA (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001). b DNA synthesis in cultures was measured using Sytox Green fluorescent dye, (mean of three biological replicates ± SEM is displayed). c Growth of M. bovis AF2122/97 was compared at 25 and 37 °C by counting colony forming units. The mean and SEM of n = 2 independent experiments (each performed in biological triplicate) is displayed. Growth was compared with day 0 by two-way ANOVA (**p < 0.01).

Back to article page