Fig. 3: M. bovis ΔespAC mutants defective in the ESX-1 machinery have impaired ability to escape from D. discoideum. | The ISME Journal

Fig. 3: M. bovis ΔespAC mutants defective in the ESX-1 machinery have impaired ability to escape from D. discoideum.

From: Mycobacterium bovis uses the ESX-1 Type VII secretion system to escape predation by the soil-dwelling amoeba Dictyostelium discoideum

Fig. 3

D. discoideum were infected at MOI 10 with M. bovis wild-type (WT) and M. bovis ΔespAC. After 3 h and 48 h, D. discoideum and extracellular bacteria were separated by differential centrifugation, and bacilli enumerated after recovery on solid medium. The experiments were performed in the absence of streptomycin; the HL5c growth medium does not support the extracellular replication of M. bovis (Fig. S2). The mean and SEM of n = 2 independent experiments (each performed in biological duplicate or triplicate) is displayed. Statistical analysis was performed by Student’s t test (*p < 0.05).

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