Fig. 1: iPolony: a polony method for quantifying virally infected cyanobacteria. | The ISME Journal

Fig. 1: iPolony: a polony method for quantifying virally infected cyanobacteria.

From: A single-cell polony method reveals low levels of infected Prochlorococcus in oligotrophic waters despite high cyanophage abundances

Fig. 1

a First, Prochlorococcus and Synechococcus are sorted based on size and their autofluorescence properties using a flow cytometer from fixed samples. b Then, thousands of sorted cells per slide are screened for the presence of intracellular viral DNA using a solid-phase PCR polony method. Percent infection is determined based on the fraction of input cells that resulted in polonies at the end of the analysis.

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