Fig. 6: Activation of ISR by a SynCom comprising four HRS-specific Firmicutes strains (HRS1–4).

a Severity of bacterial wilt disease in tomato plants inoculated with Ralstonia solanacearum after treatment with different combinations of the four selected Firmicutes bacteria. HRS1 + HRS2 mixture of Brevibacterium frigoritolerans (HRS1) and Bacillus niacini (HRS2), HRS1 + HRS2 + HRS3 mixture of HRS1, HRS2, and Solibacillus silvestris (HRS3), HRS1 + HRS2 + HRS4 mixture of HRS1, HRS2, and Bacillus luciferensis (HRS4), HRS1 + HRS2 + HRS3 + HRS4 mixture of all four Firmicutes strains. Data represent mean ± SEM (n = 12 plants per treatment). Asterisks indicate significant differences (*P < 0.05, **P < 0.01, ***P < 0.001). b, c Relative expression levels of jasmonic acid (JA) signaling marker genes (b) and salicylic acid (SA) signaling marker genes (c) in systemic leaves of tomato plants treated with the SynCom comprising all four HRS-specific strains (HRS1–4) at 0 and 12-h post inoculation (hpi) with R. solanacearum. Different letters indicate significant differences between treatments (P < 0.05; LSD test). Data represent mean ± SEM. SynCom mixture of all four Firmicutes bacterial strains, BTH 0.5-mM BTH treatment, control 2.5-mM MES buffer treatment.