Fig. 5: Occurrence of P-acquisition (PHO) genes in the genomes of plant-associated and non-plant-associated Bacteroidetes strains.

A Occurrence of all PHO genes among the 102 Flavobacterium strains. Red dots denote outliers in the data and asterisks denote significance levels (Kruskal Wallis; *<0.05, **<0.01, ***<0.001) for differences of occurrence in each group relative to the Plant-associated group. B Non-metric multidimensional scaling (Bray-Curtis distances) of P-acquisition gene content among the 102 Flavobacterium strains. Ellipses represent euclidean distances from the centre of groups (level = 0.7). C The multi-loci maximum-likelihood consensus tree was inferred from a simultaneous comparison of 10 housekeeping and core genes present in 102 Flavobacterium isolates. Tree topology and branch lengths were calculated by maximum likelihood using the VT + F + R5 model of evolution for amino acid sequences based on 1022 sites in lQ-TREE software [48]. The presence (filled symbol) or absence (hollow symbol) of selected P-acquisition genes is displayed. PstS and PhoD are highlighted in red. Subclade 1 is denoted by the numeric. Stars represent phylogenetically distinct plant-associated strains. D Occurrence of core and PHO genes among 468 Bacteroidetes strains isolated from the five environmental niches. E Occurrence of all PHO genes, excluding PitA and PhoBR, among the 468 Bacteroidetes strains. Red dots denote outliers in the data and asterisks denote significance levels (Kruskal Wallis; *<0.05, **<0.01, ***<0.001) for differences of occurrence in each group relative to the Plant-associated group.