Fig. 2: Visualization of intracellular bacteria in Bodo saltans. | The ISME Journal

Fig. 2: Visualization of intracellular bacteria in Bodo saltans.

From: Bodo saltans (Kinetoplastida) is dependent on a novel Paracaedibacter-like endosymbiont that possesses multiple putative toxin-antitoxin systems

Fig. 2

Images from Fluorescent in situ hybridisation (FISH) experiment (A–D), transmission electron microscopy (TEM) experiment (E–G) and Serial Block Face-Scanning Electron Microscopy (H, I). Imaging of B. saltans cell cultures with differential interference contrast (A), staining with DAPI to visualize nucleus and kinteoplast (B), FISH staining with endosymbiont specific probe conjugated to Cy3 (C) and images from three channels overlaid (D). Images were acquired on a Zeiss Axio Observer Z1 (Carl Zeiss AG, Jena, Germany) equipped with 100×1.4NA objective, 2.5x optovar. Images captured were analyzed using ImageJ v2.0 [94]. Ultrastructure of the B. saltans cell (E) displaying nucleus (nuc), kinetoplast (kp), mitochondria (mt), food vacuole (fv), flagellar pocket (fp) and three intracellular bacteria marked with dark red arrows, endosymbionts showing the presence of an electron lucid halo around cell membrane (F, G). 3D model of B. saltans cellular ultrastructure from two different angles (H, I). Bean shaped B. saltans cell is shown with flagellar pocket (in orange). Nucleus (in yellow) is situated in the centre of the cell and cytobionts (in blue) are distributed in close vicinity to the nucleus. A large, swirled mitochondrion (in magenta) is placed around the periphery of the cell, with a kinetoplast capsule just under the flagellar pocket. Multiple food vacuoles (in green) are present at the posterior end of the cell (colour figure online).

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