Fig. 5: UHPLC/HRMS based targeted metabolomics profiling.
From: Iron is not everything: unexpected complex metabolic responses between iron-cycling microorganisms
Targeted metabolomics used to quantify the presence of γ-butanoic acid (GABA), a degradation product in the putrescine biosynthesis pathway, in S. oneidensis MR-1 and Sideroxydans CL21 supernatant exchange experiment. Sid and Shew correspond to Sideroxydans sp. CL21 and S. oneidensis monoculture control incubations, respectively. The labeling of Sid(Sid), Sid(Shew), Shew(Shew) and Shew(Sid) are described in the legend of Fig. 3. Area under the curve of GABA in the supernatant exchange experiment was measured on day 6. Error bars represent the standard deviation of three biological replicates (n = 3). Stars indicate significance: *p < 0.05, **p < 0.01 and ***p < 0.001.
