Fig. 3: Stratification of metabolic functions along the halocline.
From: Fine-scale metabolic discontinuity in a stratified prokaryote microbiome of a Red Sea deep halocline
a Canonical correspondence analysis (CCA) for explorative reduced models (after stepwise environmental variable selection based on the AIC criterion) comparing the different samples obtained from the BSI based on the relative abundance of the different KOs belonging to the SMGC. The different samples are colored by a gradient of salinity. b Heatmap of gene abundances based on selected KEGG orthologs (KOs) in the annotated gene catalog. Metabolic functions are deduced from the profiles of selected KOs presented in Supplementary Table 2. Abundances were computed using the reads per kilobase per genome (RPKG) metric. Pathway-specific RPKG values were scaled around the means for visualization purposes as the RPKG values per pathway ranged from 0 to 32. Hierarchical clustering is based on the Euclidean distance of abundances and average linkage approach. The numerous pathways are color-highlighted based on the three broad metabolic pathways: carbon (red), nitrogen (green), and sulfur (orange).
