Fig. 1: Microbial community composition, diversity, and abundance of biocrust and topsoil samples collected across the Israel aridity gradient.

Results are shown for biocrust and topsoil samples collected for each climatic zone. a Study site showing satellite imagery basemap with country administrative borders (black dashes) and site geolocations. b Images depict (from left to right) typical site characteristics, biocrust appearance during sample collection, and soil sample appearance following simulated rainfall. c Stacked barchart showing class-level bacterial and archaeal composition based on metagenomic reads of the 16S rRNA gene. Taxonomic classification follows GTDB taxonomy. d Boxplot showing the richness estimate (Chao1) of soil and biocrust communities along the aridity gradient based on 16S rRNA gene amplicon sequencing. e Beta diversity (Bray-Curtis) PCoA ordination visualizing differences in soil and crust community composition between the four climatic zones based on 16S rRNA gene amplicon sequencing. f Boxplot showing 16S rRNA gene copy number for both crust and topsoil along the aridity gradient. Community composition based on 16S rRNA gene amplicon sequencing and richness/beta diversity based on metagenomic sequencing are shown for comparison in Fig. S2.