Table 1 Characteristic of the ten most abundant rRFs/miRNAs expressed in Rhodotorula frigidialcoholis.
rRF/miRNA ID | Size (nt) | Origin | Differential expression | Potential targets | Binding energy (kcal/mol) |
|---|---|---|---|---|---|
rRF/miRNA1 | 30 | rRNA | Not differentially expressed | ATPase GET3 | −41.6 |
Metallo-dependent phosphatase | −38.8 | ||||
Protein with homology to sperm nuclear basic protein PL-I isoform PLIb | −38.8 | ||||
rRF/miRNA2 | 18 | rRNA (28S) | Overexpressed at 0 °C | Glutathione S-transferase | −34.7 |
Unknown protein | −34.4 | ||||
Unknown protein with acetyl-CoA carboxylase/biotin carboxylase 1 domains | −32.7 | ||||
rRF/miRNA3 | 28 | rRNA (28S) | Overexpressed at 0 °C | ATP-NAD kinase | −43.2 |
Unknown protein with RNA polymerase II degradation factor 1 domain | −41.2 | ||||
Ribosome biogenesis protein ERB1 | −40.9 | ||||
rRF/miRNA4 | 29 | rRNA | Overexpressed at 23 °C | ATPase GET3 | −41.6 |
Protein with homology to sperm nuclear basic protein PL-I isoform PLIb | −39.3 | ||||
Ribosome biogenesis protein ERB1 | −36.9 | ||||
rRF/miRNA5 | 18 | rRNA (28S) | Overexpressed at 0 °C | COP9 signalosome complex subunit 7 | −34.9 |
Mannosyl-oligosaccharide alpha-1,2-mannosidase | −34.5 | ||||
RNA polymerase II degradation factor 1 | −32.9 | ||||
rRF/miRNA6 | 20 | rRNA (28S) | Overexpressed at 0 °C | RNA pol II promoter Fmp27 protein domain | −35.9 |
NAD + kinase | −35.8 | ||||
RNA polymerase II degradation factor 1 | −35.3 | ||||
rRF/miRNA7 | 22 | rRNA | Overexpressed at 0 °C | Staphylococcal nuclease domain-containing protein 1 | −33.6 |
Unknown protein | −32.8 | ||||
Glycogen synthase | −32.7 | ||||
rRF/miRNA8 | 22 | Unknown protein | Overexpressed at 0 °C | programmed cell death 6-interacting protein | −47.0 |
Kinase-like protein | −46.1 | ||||
Gutaminyl-tRNA synthetase | −45.6 | ||||
rRF/miRNA9 | 29 | rRNA (25S) | Overexpressed at 0 °C | Transcription factor IIIB 90 kDa subunit | −51.8 |
Unknown protein | −50.9 | ||||
Glycosylphosphatidylinositol inositol-deacylase | −50.3 | ||||
rRF/miRNA10 | 17 | rRNA (28S) | Overexpressed at 0 °C | COP9 signalosome complex subunit 7 | −30.5 |
26S proteasome regulatory complex component | −30.2 | ||||
DHS-like NAD/FAD-binding domain-containing protein | −28.9 |