Fig. 2: The T1-PKS and NRPS biosynthetic gene cluster necessary for ice nucleation activity (INA) in Lp VT1065. | The ISME Journal

Fig. 2: The T1-PKS and NRPS biosynthetic gene cluster necessary for ice nucleation activity (INA) in Lp VT1065.

From: Ice nucleation in a Gram-positive bacterium isolated from precipitation depends on a polyketide synthase and non-ribosomal peptide synthetase

Fig. 2: The T1-PKS and NRPS biosynthetic gene cluster necessary for ice nucleation activity (INA) in Lp VT1065.

The graphical output obtained with antiSMASH 5.0 [32] for region 2 (corresponding to the T1-PKS and NRPS gene cluster) when using the assembled genome (NCBI Accession number GCA_003049605.2) as input is shown. Prokka annotation locus IDs were added to the region overview. The start and end positions of the domains in which mutations were identified were added to the domain annotation. The approximate locations of mutations that abolished INA are indicated by red asterisks. Precise locations are listed in Supplementary Table 2. KS: ketosynthase, AT: acyltransferase, DH: dehydratase, KR: ketoreductase, C: condensation domain, A: adenylation domain, TE: thioesterase. The 4′-phosphopantetheinylation of the carrier protein domains (acyl carrier protein domain for PKS and peptidyl carrier protein domain for NRPS) are represented by the light blue ovals.

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