Fig. 6: Gut colonization by evolved plasmid-carrying bacteria, and plasmid transfer to gut-resident bacteria. | The ISME Journal

Fig. 6: Gut colonization by evolved plasmid-carrying bacteria, and plasmid transfer to gut-resident bacteria.

From: Colonization of gut microbiota by plasmid-carrying bacteria is facilitated by evolutionary adaptation to antibiotic treatment

Fig. 6

a Parallel assays of murine-gut colonization by five lines of A:H:p, C:H:p and E:H:p strains, including E:H:p strains and other RP4-carrying gut-resident bacteria in fecal samples. b Diagram of E:H:p strains transferring RP4 plasmid to gut-resident bacteria. c Plasmid RP4 recipients isolated on the last day of the colonization test were identified by Sanger sequencing of the 16S rRNA gene, including E:H:p strains, gut-resident Escherichia (E. coli, E. fergusonii), Klebsiella (K. pneumonia, K. singaporensis) and Burkholderia (B. fungorum). d No mutations were observed on RP4 plasmid from these randomly selected gut-resident bacteria. e Identification of RP4 plasmid in bacterial isolates from murine fecal samples by PCR.

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