Fig. 2: Removal of the major O-polysaccharides OBC4 and OBC5 makes P. protegens Pf-5 resistant to the tailocin #1 of P. protegens CHA0. | The ISME Journal

Fig. 2: Removal of the major O-polysaccharides OBC4 and OBC5 makes P. protegens Pf-5 resistant to the tailocin #1 of P. protegens CHA0.

From: Pivotal role of O-antigenic polysaccharide display in the sensitivity against phage tail-like particles in environmental Pseudomonas kin competition

Fig. 2: Removal of the major O-polysaccharides OBC4 and OBC5 makes P. protegens Pf-5 resistant to the tailocin #1 of P. protegens CHA0.The alternative text for this image may have been generated using AI.

a LPS profile of Pf-5 wild-type, ΔwbpL, ΔPFL_0524 and entire O-PS cluster deletion mutants. SDS-PAGE on LPS extracts was performed using a 12% acrylamide gel and components were visualized by silver staining. b Bacterial lawns of the different Pf-5 LPS mutants were exposed to extracts of the tailocin #1 of CHA0 that were serially diluted at a ratio of 1:4. As controls, Pf-5 and derivatives were also exposed to extracts containing all the phage(-related) particles extracted from CHA0, one with only the tailocin #2 and one void of any particles (Supplementary Figure 3).

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