Fig. 3: The removal of the OBC4 and OBC5 O-PS clusters makes Pf-5 resistant to the tailocin #1 in competitions with CHA0 in batch culture. | The ISME Journal

Fig. 3: The removal of the OBC4 and OBC5 O-PS clusters makes Pf-5 resistant to the tailocin #1 in competitions with CHA0 in batch culture.

From: Pivotal role of O-antigenic polysaccharide display in the sensitivity against phage tail-like particles in environmental Pseudomonas kin competition

Fig. 3: The removal of the OBC4 and OBC5 O-PS clusters makes Pf-5 resistant to the tailocin #1 in competitions with CHA0 in batch culture.The alternative text for this image may have been generated using AI.

The competitive indices (CI) of wild-type Pf-5 and LPS mutant derivatives (Δobc4, Δobc5, ΔwbpL, Δobc4Δobc5) were assessed in 1:1 ratio mixtures (a) with the CHA0 wild type (b) and derivatives Δtail2ΔmyoΔsiph producing exclusively the tailocin #1, CHA0 T#1 (c) and Δtail1ΔmyoΔsiph producing exclusively the tailocin #2, CHA0 T#2 (d). The red line indicates a competition where both strains would not be influenced by the presence of one another. Statistical differences were assessed by ANOVA and Kruskal-Wallis test using a Bonferroni correction and are indicated with letters a, b and c. Eleven biological independent experiments were performed each with three technical replicates were performed. The horizontal lines indicate the interquartile range with the center representing the median.

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