Fig. 1: Stable isotope probing (SIP) using 13C-DHP lignin polymer. | The ISME Journal

Fig. 1: Stable isotope probing (SIP) using 13C-DHP lignin polymer.

From: Discovery of lignin-transforming bacteria and enzymes in thermophilic environments using stable isotope probing

Fig. 1

A Detail of DHP lignin structure supported by GPC-MALS, 2D HSQC NMR and 13C NMR analysis. Mn number average molecular weight (average molecular weight of all the polymer chains), PDI polydispersity index or Mw/Mn ratio. Mw, Mn and PDI determined by GPC-MALS. Red dots show possible positions of 13C-isotopes in coniferyl subunit rings. B 13C-CO2 measured in the headspace of 50-ml sealed serum bottles containing 1 g (dw) of each sample incubated with 100 mg (10%) 13C-DHP lignin, determined by IR-GCMS. 13C-CO2 evolution for control bottles with no inoculum (NI) or 100 mg unlabeled 12C-DHP lignin (NC) are provided. Each point is the mean of n = 3, and error bars represent standard error. C Post-ultra-centrifugation DNA gradient in a 5 ml centrifuge tube with 13C-Low (F10), 12C-Low (F10), 13C-High (F4) and 12C-High (F6) demarcated with dashed gray lines. Graphs show DNA concentration vs. fractional density and %13C-DNA for Hog Fuel 20 cm and Lakelse samples. %13C-DNA measured using UPLC-MS/MS.

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