Fig. 2: Hog fuel MAG abundance in 13C-High (F1–4) and 12C-High (F6) SIP libraries. | The ISME Journal

Fig. 2: Hog fuel MAG abundance in 13C-High (F1–4) and 12C-High (F6) SIP libraries.

From: Discovery of lignin-transforming bacteria and enzymes in thermophilic environments using stable isotope probing

Fig. 2

Phylogenetic tree MAG placement using GTDB-TK v1.0.2 based on 120 bacterial single-copy genes. 13C-enriched genomes shown in bold. Scale shows length equivalent to 0.1 substitutions. Heatmap shows square-root transformed mean MAG abundance following DeSeq2 normalization in triplicate libraries. Abundance calculated by mapping quality-filtered reads to MAG nucleotide sequences with bbmap 38.22. Bar plot shows log2 fold change (L2FC) between 13C-High and 12C-High for each genome with >L2FC 0 indicating enrichment in 13C-High libraries. Error bars represent standard error of L2FC. Bar plot provides cut-off estimates for significance at αadj = 0.05 (individual padj values <0.05 provided).

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