Fig. 6: Aminomethyltransferase family protein phylogeny using TreeSAPP reference package. | The ISME Journal

Fig. 6: Aminomethyltransferase family protein phylogeny using TreeSAPP reference package.

From: Discovery of lignin-transforming bacteria and enzymes in thermophilic environments using stable isotope probing

Fig. 6

A Reference tree produced by TreeSAPP with 50 amino acid sequences using RAxML under the PROTGAMMALG model and 1000 iterations. Tree includes experimentally-validated vanillate/3-O-methylgalate O-demethylase (LigM) and syringate O-demethylase (DesA) proteins. B Placement of predicted MAG-encoded aminomethyltransferases into the reference tree. C Multiple sequence alignment with MAFFT using the ginsi setting under 1000 iterations for select sequences. Aromatic-binding residues derived from LigM structural model are shown in pink, folate-binding residues are shown in green, and the primary methyl-transferring catalytic tyrosine residue shown in orange. D Pathway diagram of tetahydrofolate-dependent O-demethylation of methoxylated aromatic compounds.

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