Fig. 6: Addition of MMP and serine proteases inhibitors to a bacterial consortium from lake water reduces antiviral activity. | The ISME Journal

Fig. 6: Addition of MMP and serine proteases inhibitors to a bacterial consortium from lake water reduces antiviral activity.

From: Bacterial matrix metalloproteases and serine proteases contribute to the extra-host inactivation of enteroviruses in lake water

Fig. 6

E11 (A) or CVA9 (B) were  incubated independently in the same water fraction (<0.8 μm) for 48 h at 30 °C, with and without specific protease inhibitors. GM6001 was used to inhibit MMPs, E64 was used to inhibit cysteine proteases, PMSF and Chymostatin were used to inhibit serine proteases. Experiments were conducted with three biological replicates (LW1-LW3) with two measurement points per sample. An initial titer of 106 MPN/mL was used for both serotypes. Differences in viral decay were analyzed by one-way ANOVA analysis with post-hoc Dunnett test (ns: p > 0.1, *: 0.01 < p < 0.1, **: 0.01 < p < 0.001). Numbers in italic indicate resulting p-values.

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