Fig. 3: Validation of E. faecalis transposon library screen hits.
From: Heme cross-feeding can augment Staphylococcus aureus and Enterococcus faecalis dual species biofilms

A The parental strain (OG1RF) and nine transposon mutants were grown alone (circles), or together with S. aureus (Sa, USA300LAC, triangles) for five days before biofilm levels were determined by crystal violet (CV). Data shows mean and SD relative to OG1RF-only control (N ≥ 3). **p < 0.01, ***p < 0.001 and ****p < 0.0001 when compared to respective OG1RF-only or OG1RF + Sa control biofilms using 1-way ANOVA with Dunnett’s post-hoc test. B Oxidative respiration of E. faecalis requires MenA, CydA, CydB, heme and O2.