Fig. 5: The E. faecalis cydABCD operon is required for biofilm augmentation by hemin and S. aureus, and for heme uptake. | The ISME Journal

Fig. 5: The E. faecalis cydABCD operon is required for biofilm augmentation by hemin and S. aureus, and for heme uptake.

From: Heme cross-feeding can augment Staphylococcus aureus and Enterococcus faecalis dual species biofilms

Fig. 5

A Transposon mutants for cydA, cydB, cydC, cydD and menA, deletion mutants of cydB and cydD, their chromosomally complemented strains, along with parental wild-type OG1RF, were grown alone, in the presence of hemin (25 μg/ml), or with S. aureus (Sa, USA300LAC) for five days before biofilm was quantified with crystal violet (CV). Insert shows cydABDC operon and arrows indicate the direction of transcription. B Transposon and deletion mutants for cydA, cydB, cydC and cydD, along with complementary strains and parental wild-type OG1RF, were grown overnight in the presence of hemin (5 μg/ml) before cells were pelleted, lysed and analyzed by LC–MS for intracellular heme. Data shows mean values and SD. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 when compared to respective OG1RF control (first of each group) using Tukey’s post-hoc tests (N ≥ 3).

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