Fig. 1: Vitamin storage in auxotrophic strains.
From: Metabolic adaptation to vitamin auxotrophy by leaf-associated bacteria
a Co-occurring vitamin auxotrophies as inferred from lack of growth without a given vitamin in a screen on drop-out media for 50 strains. Niacin, pantothenate, biotin, and thiamine were the most commonly required vitamins. Edge thickness correspond to the number of strains in which the two putative auxotrophies co-occur. PABA = para-aminobenzoic acid. (Source data in Source Data 1.) b Representative dynamic vitamin depletion experiments. Growth is presented as doublings over time. At t = 0, a switch from preculture supplemented with all four vitamins to a vitamin-deplete medium (coloured lines) or, as a control, supplemented medium (black line) was performed. If a strain was auxotrophic for a given vitamin, it depleted off that vitamin after some time and stopped growing; for example, Aureiomonas Leaf324 was found auxotrophic for all four vitamins. The time point at which vitamin-depleted and supplemented cultures deviated are visualized over the lineplot for all three replicates separately (see Materials and Methods for details). (Source data in Source Data 2.) c Auxotrophy heatmap. Light color refers to compounds without which a strain's growth deviates from the supplemented control and thus the strain is auxotrophic for. For example, for Aureiomonas Leaf324, all 4 depletion columns are light grey as its growth ceased without each of the compounds individually. (Source data in Source Data 3.) d Coenzyme storage in auxotrophic strains (interquartile range and median). The number of doublings in the absence of the indicated vitamin corresponds to the storage capacity. (Source data in Source Data 3).
