Fig. 2: Biotin biosynthesis pathway potential and growth characteristics of strains of the Roseobacter group with additions of biotin or desthiobiotin.

A, B Depicting biotin pathway gene presence of bacterial isolates of the respective growth experiments below (A the last four biotin genes are missing; B only bioB is present). C–H Growth experiments with the respective additions of biotin (orange, triangle), desthiobiotin (yellow, square) or no additions (gray, circle) for 6 bacterial isolates of the Roseobacter group with different biotin biosynthesis pathway potential (C, E & G biotin auxotroph; D, F & H desthiobiotin auxotroph).