Fig. 3: Growth characteristics of Vibrio campbellii and detection of extra- and intracellular biotin and desthiobiotin.

Growth of V. campbellii on glutamate without the addition of B vitamins, monitored by flow cytometer cell counts over time. Bars display extra- (blank bar) and intracellular (striped bar) biotin (orange) and desthiobiotin (yellow) concentrations during exponential, late exponential and late stationary growth phase of V. campbellii.