Fig. 1: Experimental design, fungal inoculum abundance, and soil moisture.
From: Plant-associated fungi support bacterial resilience following water limitation
a Microcosm design for H218O quantitative stable isotope probing (qSIP) assay of root-free hyphal ingrowth core soils. P. hallii plants were inoculated with either R. irregularis, S. bescii, or left uninoculated (indicated in yellow, blue, or black, respectively) and grown under water-replete or water-limited conditions (indicated in dark or light shades and solid or dashed lines, respectively; n = 3 replicates per treatment). After 3 months, hyphal ingrowth core soils were amended with enriched (H218O) or natural abundance (H216O) water and incubated for 7 days. b Abundance of R. irregularis and (c) S. bescii (DNA copies g−1 soil) measured with strain-specific qPCR primers after 3 months. d Soil moisture after 3 months. Bold lines represent median value; whiskers represent upper and lower quartiles (n = 6 replicates per fungal*moisture treatment combination). Asterisks denote the results of a nonparametric Kruskal–Wallis rank sum test performed separately for each qPCR plot (p < 0.001). Letters denote a Tukey’s HSD test for soil moisture comparisons (p < 0.001).
