Fig. 6: Community response to the coevolved ciliate predator is contingent upon coevolved Pseudomonas SBW25.

Heatmaps for genes identified as differentially expressed [39] at day 4 (n = 1143) in response to Pseudomonas coevolution contingent up coevolved ciliate predation while controlling for the overall effects of Pseudomonas SBW25 coevolution and coevolved ciliate predation. Each row is a gene colored by species identity and each column is a sample, and columns are colored according to treatment (see Fig. 1 in main text). Heatmap color shows the Z-score for each gene across treatment categories. The scatterplot shows the estimated log₂ fold-change of transcripts (points) from ancestor to coevolved Pseudomonas SBW25 treatments contingent upon the presence of the coevolved ciliate (i.e., the light green to dark green column in the heatmap). Genes are only included if they were assigned to KEGG [45] functional pathways that are over-represented in the list of differentially expressed genes from each species using the hypergeometric test. KEGG pathways are shown on the horizontal axis and point size is proportional to the normalized mean expression (Mean expr) level across all treatment conditions.