Fig. 3: A distinction between conjugation efficiency and transconjugant abundance for conjugative plasmids when selecting for private vs. public antibiotic resistance.
From: Cooperative antibiotic resistance facilitates horizontal gene transfer
A Relative differences (IMP/AB-free) in abundances of non-producer populations (N) when cultivated with the different producers [Pchr], [Pconj] and [Pnon-conj] in the presence of imipenem (IMP) and without antibiotics (AB-free), started at different initial producer to non-producer ratios (100:1, 1:1, and 1:100). B Conjugation efficiencies (\(\frac{{{{{{{{\boldsymbol{T}}}}}}}}}{{{{{{{{{\boldsymbol{T}}}}}}}} + {{{{{\boldsymbol{N}}}}}}_{{{{{{{\mathbf{{rem}}}}}}}}}[{{{{{\boldsymbol{P}}}}}}_{{{{{{{\mathbf{{conj}}}}}}}}}]}}\)), calculated for Pconj co-cultures exposed to imipenem (IMP), tetracycline (TET), or without antibiotics (AB-free). C Transconjugant abundances (\(\frac{{{{{{{{\boldsymbol{T}}}}}}}}}{{\sqrt {{{{{{{{\boldsymbol{P}}}}}}_{{{{{{{\mathbf{conj}}}}}}}}}} \times \left( {{{{{{{{\boldsymbol{T}}}}}}}} + {{{{{{{\boldsymbol{N}}}}}}_{{{{{{{\mathbf{rem}}}}}}}}}}\left[ {{{{{{{{\boldsymbol{P}}}}}}_{{{{{{{\mathbf{conj}}}}}}}}}}} \right]} \right)} }}\)), calculated for Pconj co-cultures exposed to IMP, TET, or AB-free. For (A), (B), and (C), dots represent individual data points (n = 6), and lines connect the mean for co-cultures started at different initial producer to non-producer ratios and incubated for 24 h. Shaded areas are 95% confidence intervals of the mean. The skull and crossbones symbols indicate that no transconjugants were identified in 100:1 samples exposed to TET.
