Table 1 Overview of the sample numbers at various stages of the experiment and analysis.
Method | Type | Samples (n) | Information |
|---|---|---|---|
Field sampling | Sediment cores | 11 | Per sampling site |
66 | Total cores taken | ||
18 | Zero time point | ||
BW (DNA) | 66 | 16S rRNA gene amplicon sequencing | |
SED (DNA) | 66 | 16S rRNA gene amplicon sequencing | |
SED (RNA) | 24 | Metatranscriptomics | |
Chemistry | 66 | Various chemical parameter analysis | |
Temperature | Annual mean | 6 | 3 × 2 bays water column temperature |
Incubation mesocosm | 6–35 °C | 48 | 3 × 2 bays for 8 different incubation temperatures |
Core height | SED | 48 | SED height measurements |
BW | 48 | BW height measurements | |
BP | BW | 150 | 3 sites × 2 bays × 3 days × 8 temperatures × 6 zero time points |
Alpha diversity | BW (ASVs) | 66 | |
SED (ASVs) | 65 | ||
Chemistry | Various BW or SED | 66 | If not otherwise stated, all samples were included in the analysis |
BW salinity | 63 | ||
SED Fetot | 65 | ||
Db-RDA | BW | 65 | |
SED | 65 | ||
nMDS | BW | 66 | |
SED | 65 | ||
Differential abundance analysis | BW | 24 | Within mesocosm comparison 3 × 2 per bay compared to 7 temperatures |
48 | total | ||
SED | 11 | 3 per sampling site compared to 8 temperatures (for each site) | |
48 |
