Fig. 2: Sequence logos illustrating conservation of amino acid residues across the α-amanitin MSDIN sequences of Lepiota venenata, Galerina marginata, and Amanita phalloides (top) as compared to an alignment of representative sequences from the 43 unique MSDIN core sequences found within A. phalloides (bottom).

Larger letters in the sequence logo correspond to a greater frequency of the specific amino acid residue at a given site. We inferred selection acting on leader, core, and follower portions of MSDINs and across entire sequences using dN/dS ratios (displayed above logos) as calculated in PAML. We were unable to measure selection acting on A. phalloides core sequences (bottom, middle) because informative sites are saturated across this highly diversified region.