Fig. 4: The ET-Nod governing nodulation differs according to the strain. | The ISME Journal

Fig. 4: The ET-Nod governing nodulation differs according to the strain.

From: Widespread Bradyrhizobium distribution of diverse Type III effectors that trigger legume nodulation in the absence of Nod factor

Fig. 4: The ET-Nod governing nodulation differs according to the strain.

Images of roots and nodules of A. indica plants at 21 days post inoculation with strain USDA61 (A) or ORS86 (B) and their respective mutants in T3SS (ΩrhcJ or ΩrhcN) and several identified ET-Nods (ΩernA, Ωsup3, Ωbel2-5) (scale bars: upper 0.5 cm, lower 0.2 cm). Nodule number on A. indica plants at 21 days after inoculation with USDA61 (C) or ORS86 (D) and their respective mutants. Box plots show the results of one of the two experiments performed independently (8 plants each). *p < 0.05, **p < 0.005, ***p ≤ 0.0005, ****p < 0.0001, significant differences between the wild-type strain and each mutant using a nonparametric Kruskal–Wallis test, NS not significant. The mutants USDA61ΩernA, USDA61ΩrhcJ and USDA61Ωbel2-5 were previously obtained [12, 62, 63].

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