Fig. 5: The rpoB-normalized transcript abundance of core metabolism pathway genes in six major AOA lineages (Nitrososphaeraceae, NS-beta-associated, NS-delta, NS-beta, NS-epsilon, and the order-level lineage Nitrosopumilales) from EAA soils. | The ISME Journal

Fig. 5: The rpoB-normalized transcript abundance of core metabolism pathway genes in six major AOA lineages (Nitrososphaeraceae, NS-beta-associated, NS-delta, NS-beta, NS-epsilon, and the order-level lineage Nitrosopumilales) from EAA soils.

From: Nitrogen and phosphorous acquisition strategies drive coexistence patterns among archaeal lineages in soil

Fig. 5: The rpoB-normalized transcript abundance of core metabolism pathway genes in six major AOA lineages (Nitrososphaeraceae, NS-beta-associated, NS-delta, NS-beta, NS-epsilon, and the order-level lineage Nitrosopumilales) from EAA soils.The alternative text for this image may have been generated using AI.

Normalized transcript abundances of three pure culture strains during ammonium-replete (Nitrososphaera viennensis EN76 and Nitrosopumilus maritimus SCM1) or urea-replete (Ca. Nitrosopelagicus brevis U25) exponential growth from previous studies are given for comparison [48, 51, 52]. The cell diagram shows the core AOA metabolic pathways and proteins for ammonia transport, oxidation and assimilation, electron transfer, HP/HB carbon fixation, and ATP synthesis. The heatmap shows log2-transformed ratios of target gene transcript abundance to rpoB transcript abundance within each lineage averaging different plots and seasons. Cross mark “X” indicates gene absent in a lineage or strain; “nd” indicates gene present, but no transcripts detected in metatranscriptomes. Red arrows denote electron flow and dashed arrows indicate proposed pathways yet to be experimentally verified. AmoABCXYZ ammonia monooxygenase subunit A, B, C, X, Y, and Z, NirK copper-containing nitrite reductase, AccB acetyl-CoA carboxylase beta, Msr Malonic semialdehyde reductase, 4HBD 4-hydroxybutyryl-CoA dehydratase, CoxA Cbb3-type cytochrome c oxidase subunit I, PetB putative cytochrome b/b6 domain protein, Amt-1 and Amt-2 type 1 and 2 ammonium transporters, SSS-1 and SSS-2 type 1 and 2 sodium-solute symporters, Ut urea transporter, UreC urease subunit alpha, GlnB nitrogen regulatory protein P-II, PstAC phosphate uptake transporter permease protein A and C, PstB phosphate transporter ATP-binding protein, PiT low affinity inorganic phosphate transporter, AtpA ATP synthase subunit A, FlaB archaeal flagellin.

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