Fig. 1: Principle behind the chromosomally-integrated inducible mutator strain. | The ISME Journal

Fig. 1: Principle behind the chromosomally-integrated inducible mutator strain.

From: Impact of transient acquired hypermutability on the inter- and intra-species competitiveness of Pseudomonas aeruginosa

Fig. 1

In step 1, a mutS transgene was introduced under the control of a rhamnose-inducible promoter at a neutral site in the PAO1 chromosome. In step 2, the endogenous mutS gene was deleted. This yielded a strain where, in the absence of inducer (rhamnose) mutability was high (due to low intracellular MutS levels) whereas in the presence of inducer, mutation is suppressed.

Back to article page