Fig. 3: Phylogeny of lineage IV Nitrospirales based on 120 conserved proteins.

Maximum likelihood tree based on a concatenated alignment of 120 conserved bacterial proteins from representative lineage IV Nitrospirales genomes and MAGs. The newly cultured marine Nitrospirales described in this study are highlighted in red. Isolated or enriched organisms are indicated by full and open circles, respectively. Please refer to Materials and Methods and Table S2 for details of the sequence dataset used to calculate this tree. The tree is excerpt from a more encompassing tree of the Nitrospirales (Fig. S3). The Nitrospirales outside of lineage IV are used as outgroup. Numbers on the branches indicate ultra-fast bootstrap support (n = 1000). Asterisks indicate a bootstrap value of 100. Sample sources are shown in parentheses. The scale bar shows 0.1 estimated substitutions per amino acid.