Fig. 1: Growth of Delftia sp. DT-2 and Bacillus sp. BP-3 in mono- or co-culture.
From: Two-tiered mutualism improves survival and competitiveness of cross-feeding soil bacteria
The two strains were grown separately or together on ST10-1 agar plates (A) or in ST10-1 liquid medium (B). Effects of the culture supernatant of DT-2 on the growth of BP-3 on ST10–1 agar plates (C) or in ST10-1 liquid medium (D). Effects of the culture supernatant of BP-3 on the growth of DT-2 on ST10–1 agar plates (E) or in ST10–1 liquid medium (F). Ten mL aliquots of the filtered culture supernatant were mixed with 10 mL fresh ST10–1 liquid culture medium for inoculation of BP-3 and DT-2. The control group consisted of fresh ST10–1 liquid culture medium without supernatant. The strains were grown in the treated culture medium for two days. The data are presented as the mean ± standard error (n = 3–4). *** indicates significant differences between treatments at p < 0.0001 (Tukey’s test).
