Fig. 6: Microscopic analysis of biofilms from the A. chroococcum HN and P. aminovorans HPD-2 monocultures and co-culture.

MIX—co-culture of A. chroococcum HN and P. aminovorans HPD-2, HN—monoculture of A. chroococcum HN, HPD-2—monoculture of P. aminovorans HPD-2. a Scanning electron micrographs of biofilms from the monocultures and co-culture and EDS analysis of the bridge-like structure observed in the co-culture biofilm. b Confocal laser scanning image of FISH-stained biofilm planes of A. chroococcum HN and P. aminovorans HPD-2 from the monocultures and co-culture. Images from left to right include the monoculture of A. chroococcum HN, monoculture of P. aminovorans HPD-2, and co-cultures of A. chroococcum HN and P. aminovorans HPD-2. Red represents A. chroococcum HN, green represents P. aminovorans HPD-2, and blue represents the nucleus. c, d Confocal laser scanning image of FISH-stained biofilm 3D structure from monocultures and co-culture of A. chroococcum HN and P. aminovorans HPD-2 with or without the blue of the nucleus.