Fig. 5: Effect of ecology on vitamin, cofactor, and amino acid metabolism gene fitness.
From: Mutualism reduces the severity of gene disruptions in predictable ways across microbial communities
A Normalized gene fitness scores for representative genes involved in biosynthesis. B Final OD600 of wild type and mutant S. enterica (S) cultivated in fresh galactose media (left), E. coli (E) spent media (middle), and M. extorquens (M) spent media (right). All experiments were performed in triplicate. C S. enterica ∆ilvA mutant (left) and ∆panC mutant (right) normalized yield on galactose and succinate minimal media with varying amounts of supplementation. Note that the y-axis units – relative final density – is the OD600 at a specified nutrient concentration supplemented into the medium divided by the maximum OD600 for each supplemented nutrient. The x-axis is square root transformed. All experiments were performed in triplicate. D Final OD600 of mutants ∆ilvA and ∆panC S. enterica grown in spent media prepared from wild type S. enterica grown to mid-log phase in galactose (left) or succinate (right) minimal media. All experiments were performed in triplicate. For all of the absorbances shown (B–D), the absorbance of the medium blanks were subtracted from the experimental treatments’ absorbance values.
